🧬 Osteosarcoma Cell Lines

Reference information for osteosarcoma cell lines that are likely similar to Sid's cancer cells.

HOS is a fibroblast and epithelial-like cell line that was isolated from the bone of a White, 13-year-old, female patient with osteosarcoma. This cell line was deposited by JS Rhim.

Morphology: fibroblast and epithelial-like
Tissue: bone / osteosarcoma
Patient: 13yr-female

Genomics

  • TP53 likely loss of function homozygous mutations (recapitulates TP53 LOH)
  • CDKN2A homozygous deletion
  • Few major additional mutant genes

Curated Citations

  • Rhim JS, et al. Non-producer human cells induced by murine sarcoma virus. Int. J. Cancer 15: 23-29, 1975. PubMed
  • McAllister RM, et al. Cultivation in vitro of cells derived from a human osteosarcoma. Cancer 27: 397-402, 1971. PubMed
  • Rhim JS, et al. Characterization of non-producer human cells induced by Kirsten sarcoma virus. Int. J. Cancer 16: 840-849, 1975. PubMed
  • Rhim JS. Characterization of sarcoma-positive, leukemia-negative (S+L-) human cells induced by the feline leukemia virus pseudotype of Moloney sarcoma virus. Proc. Soc. Exp. Biol. Med. 167: 597-606, 1981. PubMed
  • Rhim JS, et al. Differential susceptibility of human cells to transformation by murine and avian sarcoma viruses. Proc. Soc. Exp. Biol. Med. 170: 350-358, 1982. PubMed

U-2 OS is a cell line with epithelial morphology that was derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15-year-old, White, female osteosarcoma patient.

Morphology: epithelial
Tissue: bone / osteosarcoma
Patient: 15yr-female

Genomics

  • Chromosomal altered
  • No coding mutations in CDKN2A, TP53, RB1, PTEN, BRAF or in various COSMIC genes

Curated Citations

  • Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed
  • Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed
  • Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159 : 531-541 , 1994. PubMed
  • Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed
  • Moradpour D, et al. Characterization of cell lines allowing titghtly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996. PubMed

Established from a chemotherapy-resistant tumor sample obtained from the left knee of a 10-year-old boy with anaplastic osteoblastic sarcoma (Rosen grade IIB) in 1989.

Morphology: fibroblastoid cells
Tissue: bone / osteosarcoma
Patient: 10yr-male

Genomics

  • No major cosmic driver mutations

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